LEPTOSPIRILLUM FERROOXIDANS PDF

Gene function analysis in extremophiles: the "nif" regulon of the strict iron oxidizing bacterium " Leptospirillum ferrooxidans ". In Centro de Astrobiologia it has been considered the Tinto river as a model ecosystem to study life based on iron. The final goal is to study the biological and metabolic diversity in microorganisms living there, following a genomic approach, to get insights to the mechanisms of adaptation to this environment. The Gram-negative bacterium Leptospirillum ferrooxidans is one of the most abundant microorganisms in the river, and it is one of the main responsible in maintenance of pH balance and, as a consequence, the physico-chemical properties of the exosystem. We have constructed a Shotgun DNA microarrays from this bacterium and we have used it to studied its genetic capacity for nitrogen fixation. With this approach we have identified most of the genes necessary for dinitrogen N2 reduction, confirming the capacity of L.

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The importance of Leptospirillum ferrooxidans for leach processes has been evaluated by studying the lithotrophic flora of three mine biotopes and a heap leaching operation, by percolation experiments with inoculated, sterilized ore, and by morphological, physiological, and genetic investigations of pure and mixed cultures of L. Leptospirilli represent at least one-half of the ferrous-iron-oxidizing population. Percolation experiments confirmed this result. Leptospirilli were as numerous as T.

At reduced temperatures, the generation times of leptospirilli increase more so than those of T. Physiological investigations indicate that L. Even an addition of 0. The maximum ferrous-iron-oxidizing activity of L.

After growth on sulfidic ore, both species exhibit reduced iron-oxidizing activities, L. Surprisingly, the absolute values are similar. For indirect leaching, L. This was confirmed by the results of percolation experiments. The best results were obtained with a mixed culture of all three species.

National Center for Biotechnology Information , U. Journal List Appl Environ Microbiol v. Appl Environ Microbiol. Author information Copyright and License information Disclaimer.

Copyright notice. This article has been cited by other articles in PMC. Abstract The importance of Leptospirillum ferrooxidans for leach processes has been evaluated by studying the lithotrophic flora of three mine biotopes and a heap leaching operation, by percolation experiments with inoculated, sterilized ore, and by morphological, physiological, and genetic investigations of pure and mixed cultures of L. Images in this article Image on p.

Image on p. Ahonen L, Tuovinen OH. Microbiological oxidation of ferrous iron at low temperatures. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. J Bacteriol. Microorganisms in reclamation of metals.

Annu Rev Microbiol. The taxonomy of certain thiobacilli. J Gen Microbiol. Deoxyribonucleic acid homologies among 96 strains of ammonia-oxidizing bacteria. Arch Microbiol. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.

J Mol Biol. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. Icosahedral inclusions carboxysomes of Nitrobacter agilis.

Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0. Associated Data Supplementary Materials. Support Center Support Center. External link. Please review our privacy policy.

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Leptospirillum

Systems used to automatically annotate proteins with high accuracy:. Select item s and click on "Add to basket" to create your own collection here entries max. Leptospirillum is a genus of iron-oxidizing bacteria which plays an important role in industrial bioleaching the conversion of metals to a soluble form and biooxidation the extraction of metals. They are obligate aerobes. Leptospirilla have been found to be the primary iron-oxidizers in industrial continuous-flow biooxidation tanks probably because the high ferric-ferrous iron ratio inhibits all but species of Leptospirillum. Leptospirilla are also important contributors to the acid mine drainage process; these combine to make Leptospirilla more important players in acid mine processes than previously thought.

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A Microbial Biorealm page on the genus Leptospirillum. Leptospirillum is a genus of iron-oxidizing bacteria which play an important role in industrial bioleaching the conversion of metals to a soluble form and biooxidation the extraction of metals. They are obligate aerobes require oxygen gas. Leptospirilla have been found to be the primary iron-oxidizers in industrial continuous-flow biooxidation tanks. The reason for this bacteria's domination in biooxidation is most likely the fact that the high ferric-ferrous iron ratio inhibits all but species of Leptospirillum. Leptospirilla are also important contributors to the acid mine drainage process; these combine to make Leptospirilla more important players in the acid mine processes than previously thought.

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